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Treatment with sulphuric acid led to perfect germination in a well-ripened sample of seed, and the method was used for many years and is, I believe, still used by the Bihar planters.
In most cases, DNA was prepared from plants grown from a random bulked sample of seed.
For each species, a mixed sample of seed from at least 100 individuals randomly chosen from the whole site was collected.
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A sample of seeds was analyzed using two mass spectrometers connected by a chamber called a collision cell, in which the seeds' molecules were shattered into fragmentation ions.
The collectors follow internationally recognized protocols for gathering a genetically diverse sample of seeds within each species.
In his stunning Seed Cathedral (above) for Shanghai's 2010 World Expo, the Union Jack is there, but you'll find it shrouded in the building's 66,000 fiber-optic rods, each embedded with a sample of seeds at the tip supplied by the Kew Royal Botanic Gardens (who gave 250,000 seeds in all).
The latter was determined on a sample of seeds, one spike in the glasshouse or 1,000 seeds from all spikes in each 1×1.08 m plot in the field.
Advantages of the genetic over the somatic and molecular approaches are nondestructive testing and applicability to a large sample of seeds.
Of the 11 germination experiments, 9 had >90% germinated or nongerminated seeds genotyped with this marker-genotyping system, and the success rate was similar to that (93%) for a random sample of seeds segregating for SD 12 (Table 3).
The heterogeneity occurs in a sample of seeds from a pure line (due to nongenetic factors) and a segregating (e.g., F2) population (due to both genetic and nongenetic factors).
Although tetraploids were not obtained in this experiment, probably since the sample of seeds germinated was too small, we could reasonably assume that their most likely primary origin in the wild would be BSP via the mating of two unreduced gametes produced by diploids.
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