In the present study, we used Solexa sequencing technology on small RNA libraries prepared from a mixture sample of rat ischemic cortex at 5 time points (3, 6, 12, 24 and 72 h) of reperfusion after FNS.
All sequences were aligned by using MUSCLE in MEGA 5.2 (http://www.megasoftware.net/), manually inspected for consensus, and compared with the 267-bp fragment generated from the known sample of rat lungworm.
For determination of complement anaphylatoxin C5a levels in plasma samples of rats, an ELISA-system was developed.
After euthanasia, blood and liver samples of rats were collected for histological and biochemical analyses.
Urine samples of rats from all groups were subjected to qualitative analysis of nitrates, pH, protein, glucose, ketone, urobilinogen, and bilirubin.
Samples of rats taken from the healed abdominal wall and the healed cecal wall showed integral neo-mesothelial cells layers, with various fibrosis (Figs. 4D and 4E).
Blood samples of rats were centrifuged at 2,000 g for 10 minutes at 4°C and aliquoted for the respective analytical determinations.
In short, samples were boiled in ethanol and assayed with a commercial kit (ImmuChem Double Antibody Corticosterone I RIA, MP Biomedicals, CA, USA) intended for the analysis of plasma and extracted urinary samples of rats and mice.
Hydroxylated BDEs (OH-BDEs) have been identified in environmental samples in several studies [ 58– 60] and have been observed in serum samples of rats dosed with PBDEs [ 61], suggesting they are products of PBDE metabolism.
