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The sample of pneumococcal disease in children studied is therefore likely to be representative of the incidence of disease in this area.
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During the follow-up between September 2001 and May 2002, nasopharyngeal (NP) samples of pneumococcal carriage were collected from the study participants at 10 monthly visits.
Of these, 99 (55.3%) patients provided a urinary sample for pneumococcal antigen detection, 37 (20.7%) a sputum sample, and 48 (26.8%) blood cultures.
JB organized the gathering of pneumococcal samples at Herlev Department of Clinical Microbiology.
JDK and CØ organized the gathering of pneumococcal samples at Hvidovre Department of Clinical Microbiology.
HCS participated in the design of the study, organized the gathering of pneumococcal samples at Aalborg Department of Clinical Microbiology, and helped to draft the manuscript.
Seven serotypes of 23-valent pneumococcal vaccine were chosen as a representative sample of differing immunogenic strengths of pneumococcal vaccine.
To better understand the population impact of these vaccines, comprehensive analysis of large collections of pneumococcal isolates sampled prior to vaccination is required.
Direct detection and subsequent serotyping on clinical samples will improve the accuracy of pneumococcal surveillance to monitor vaccine effectiveness.
Methodology of sampling, isolation, identification and serotyping of pneumococcal strains, as well as susceptibility testing is mentioned elsewhere in detail [ 9].
In a sandwich or inhibition version of the first described pneumococcal Luminex assay, also detection of pneumococcal polysaccharides in urine samples appeared to be possible [ 10, 32, 33].
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