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The mapping of the forward and reverse reads indicates the sample of origin of the original cluster and was used to measure demultiplexing misassignments rates.
Briefly, we compute the likelihood of a read to originate from each of the original samples, assign reads to the most likely sample of origin and compute the overall confidence in this assignment.
To identify the sample of origin for rock clones, cloned inserts were amplified with a fluorescently labeled forward primer.
To exclude the possibility that this contamination biased the identification of PKS I proteins, the sample of origin of each of protein identified was examined.
The tagged primers also allowed a posteriori attribution of most reads to their sample of origin.
The computational process by which reads are assigned to the sample of origin is called demultiplexing.
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Small numbers to the left of each gray bar indicate the samples of origin.
Let M be the number of potential samples of origin and let Z 0 1, Z 0 2, … , Z 0 M be the likelihood scores for each sample.
We compute the likelihood of assignment of a read to all potential samples of origin, assign each read to the most likely sample and compute the uncertainty of the assignment.
In the CAM sample, country of origin was determined in 458 (93.5%) of 497 assessed studies.
We examined 15 single-nucleotide polymorphisms (SNPs) in DRD3 in our sample of European origin consisting of 95 small nuclear SCZ families and 167 case control pairs.
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