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A sample of muscle tissue from each specimen was extracted using an automated Glass Fiber protocol [11].
All specimens were deposited in the MLBM fish collection as vouchers at the completion of this study, and all specimens also have a sample of muscle tissue stored in the LVT collection at UNLV.
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Prior to sampling of muscle tissue, fish were killed in an overdose of MS-222.
We also analyzed 4 samples of muscle tissue from the zone adjacent to the examined epidermis.
Their ethnicity was Japanese.We also analyzed four samples of muscle tissue from the zone adjacent to the examined epidermis.
Samples of muscle tissue from nine male pilot whales were obtained from specimens harvested in 2006 and archived by the Faroese Museum of Natural History.
From each individual, several samples of muscle tissue from the central part of the body (10 g wet weight each; ww) were removed in order to conduct all the measurements for pollutants.
After assessment of the fast twitch and tetanic contraction capacity of the muscle at days 4 and 14 post injury, sampling of muscle tissue served for analysis of cell apoptosis (cleaved caspase 3 immunohistochemistry), cell proliferation (BrdU immunohistochemistry) as well as of muscle tissue area and myofiber diameter (HE planimetric analysis).
We next analyzed the TRF lengths of the HinfI digested-DNA samples of muscle tissues from 4 WS patients (shown in Table 1) and control patients (shown in Table S2).
Contemporary samples consisted of muscle tissues donated by hunters and trappers, collected from roadkills or taken from live-trapped individuals during previous research (e.g. Harveson 1997).
On the basis of those experiments, a 1D measurement was established to allow correct estimation of the BV/TV of a TB sample also in the presence of muscle tissue in the sensitive volume of the NMR-scanner.
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