It was assessed by assaying samples of murine serum and lung tissue sample, at three different concentrations, during the same day and three different days under the same experimental conditions.
In this study, we presented evidence for abundant expression of oncofetal fibronectin (containing the alternatively spliced EDA and EDB domains) in the samples of murine sarcomas and human specimens.
Because of these reasons we used genome-wide expression profiling analysis to investigate differences among samples of murine fibroblasts carrying Kras codon 12 or 13 mutations and cells that constitutively overexpress the wild-type (wt) Kras gene.
Here we report the results of phylogenetic analyses of the endemic African murines through a broad sampling of murine diversity from all their distribution area, based on the mitochondrial cytochrome b gene and the two nuclear gene fragments (IRBP exon 1 and GHR).
Accordingly, Figure 2(c) shows the electrophoretic analysis of PCR products obtained by the amplification of four samples of murine genomic DNA with primers HuBetaF and HuBetaR (Table 1), specific for the human β-globin gene: again, the expected 449 bp band was generated only by the amplification of genomic DNA belonging to the founder mouse TG1.
As a guide to which innate immune genes we might expect to detect in single macrophages, we used real-time PCR to determine the relative expression of a panel of genes in a 2.1×106-cell sample of primary murine bone marrow-derived macrophages (BMDM) under resting and stimulated conditions and then compared their expression to that of EF1α (Figure 4).
The conditions for the Leishmania PCR were optimised and a standard curve of serial diluted samples, consisting of murine tissue mixed with known quantities of L. donovani, showed a linear signal between 1 and 4×105 parasites in a 80 µl sample.
Via confocal sampling of the murine outer ear, Raman spectra were obtained at multiple depths.
Samples of unlabelled murine CARD9 CARD and N-labelled human NOD2 (28–218) were buffer exchanged overnight into 20 mM sodium phosphate (pH 7.1), 100 mM NaCl and 5 mM DTT.
NP carried out preparation of murine samples for microarray analysis.
