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A modified aerospray apparatus was used to prepare a thin layer sample of matrix and analyte for quantitative analysis by MALDI-TOFMS.
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A sample of matrices of the study shown in the data matrices were used to enable systematic comparison [ 26].
The average MAS5-calculated signal intensity of replicate samples of matrix, ORS, DF, DP, and melanocyte cells was determined for each gene/probe, and presented as a relative percent value versus the average ORS probe signal intensity.
This procedure is based on the determination of calcium by calculating the difference in fluorescence intensity of calcium bound Fura-2 in triton-lysed samples of matrix vesicles that were incubated for 24 h in synthetic cartilage lymph vs. fluorescence intensity of Fura-2 bound calcium in triton-lysed samples of freshly isolated, non-incubated matrix vesicles (control).
These samples were considered as post-extracted samples (presence of matrix).
A sample of metagenomic matrix and network were illustrated in Figure 1 and the similarity matrices of all datasets were shown in Additional file 2: Table S1.
bSpiked matrix number of samples consisting of matrix with nontarget DNA only; field samples number of samples that generated melting curves different from those of target DNA.
A sample of decellularized matrix was frozen in Tissue Tek O.C.T. freezing medium, sectioned into 10 µm slices, and stained with hematoxylin and eosin (H&E) to confirm the absence of cells.
Similarly, the aqueous solution of individual analyte either at LQC or HQC level was prepared with the elution solvent and was considered as aqueous samples (absence of matrix).
The principles and characteristics of sampling and analysis of different types of sample matrix are discussed, i.e., of air, water, biological, organic, and soil matrices.
While the CS framework requires a particular form of sampling (incoherent sampling), the related paradigm of low-rank matrix recovery (MC) assumes a random sampling of the matrix entries.
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