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A convenience sample of lung cancer patients was recruited from chest medicine and oncology inpatient units at three teaching hospitals in the Taipei area of Taiwan.
To determine the sites of APMV replication, one half of each sample of lung, nasal turbinate, brain, spleen, kidney and small intestine tissue collected 3 dpi and 14 dpi was homogenized and clarified supernatants were prepared.
Cores of tissues of 0.6mm diameter in 3 to 4 replicates were arrayed on paraffin blocks, using normal lung, liver and gastrointestinal tract tissues as control, and a constant sample of lung carcinoma for normalization across different tissue array blocks.
However, positive antibodies against Coccidioides spp. were found in 1 sample of lung from Glossophaga soricina bats.
After correcting for the protein concentration of each sample of lung tissue, SDS-polysacrylamide electrophoresis was performed.
We also analyzed a tissue sample of lung metastasis of colorectal cancer obtained by needle biopsy from a surgically resected lung tumor.
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So far they've included samples of lung cancer, leukemia, breast cancer, and liposarcomas.
48 h later the animals were sacrificed and samples of lung tissue, bronchoalveolar lavage and blood were collected.
The method was used for analysis of adenosine in urine samples of lung cancer patients and its accuracy was evaluated by comparison of the results of the proposed method with the standard method of HPLC-UV.
Moreover, the present platform was successfully applied to the determination of AD in urine samples of lung cancer patients and the range of the recoveries was from 90% to 106%.
For the random sputum samples of lung cancer patients, the detection rate was 47.5%, but in the induced sputum, BAL and pleural fluids, the detection rate was up to over 70.0%.
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