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All breast cancer cases and a selected sample of benign breast diseases were confirmed pathologically.
A section of tumour tissue and a sample of benign mucosa from the same organ were collected from each patient.
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In samples of benign prostatic hyperplasia (n=10), no FGF8b expression was detected.
Additional portions of thyroid tumours and samples of benign disease were frozen at −80°C for later Western blot analysis.
Samples of benign naevi and metastatic melanomas for RNA isolation were obtained from 2-mm diameter cores of FFPE blocks.
There was no significant difference in COX-2 positivity between samples of benign and borderline tumours (Table 1).
We investigated the apoptotic profile of salivary neoplasms in twenty fresh samples of benign and seven samples of malignant salivary neoplasms, using quantitative real time PCR.
There were six samples from tumour grade 1, seven samples each from grades 2 and 3, and five samples of benign breast tissue as the control population.
In addition, 39 samples of benign prostatic hyperplasia (BPH) patients (59 76 years of age; mean 68 years), who underwent transurethral resection of the prostate, were included.
There was no significant difference in the levels of VEGF-C, VEGF-D and VEGFR-3 positivity between samples of benign and borderline tumours (Table 1).
Biochemical and immunochemical methods were used for cross-linked telopeptides of type I and III collagens in samples of benign and malignant serous tumours.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com