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Sample mix up issue.
2.2 Sample mix up issue The first and the fore most step in suspicion of a mixed profile if any is to evaluate the DNA profile to its entirety.
If a standard singleplex PCR approach had been used, there would be a strong suspicion of artificial recombination via sample mix up.
To avoid sample mix up, each individual was processed separately.
To exclude sample mix up by design, we have set up 2 liquid handlers with barcode reading capabilities.
The laboratory may choose to add a disclaimer which addresses general pitfalls in laboratory testing such as sample quality and sample mix up.
Two birds from the LWS line were excluded from the analysis as results from a phylogenetic analysis indicated that there had been a sample mix up.
In addition, in light of the finding that sample mix up is a common problem in cancer genetic studies (here illustrated by two mismatched pairs out of 96), we have recognized the need for robust and scalable identification methods.
If there is a sample mix-up, the measurements will not correspond to the correct samples.
Moreover, the MC-MHS-PCR is very rapid, easily implemented, straightforward, and minimizes the risk of sample mix-up and laboratory contamination.
No evidence of sample mix-up or contamination was observed (data not shown).
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