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To avoid sample mix up, each individual was processed separately.
In addition, in light of the finding that sample mix up is a common problem in cancer genetic studies (here illustrated by two mismatched pairs out of 96), we have recognized the need for robust and scalable identification methods.
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If there is a sample mix-up, the measurements will not correspond to the correct samples.
Moreover, the MC-MHS-PCR is very rapid, easily implemented, straightforward, and minimizes the risk of sample mix-up and laboratory contamination.
No evidence of sample mix-up or contamination was observed (data not shown).
There is no reason to assume that other databases, e.g. for animal mtDNA, are less prone to sample mix-up.
For the time being, one has to anticipate an omnipresent thin layer of artificial variation incurred by sample mix-up.
The sequences showed a patient-specific clustering with high bootstrap support (>90%), which argues against the occurrence of contamination and sample mix-up (data not shown).
Fourth, due to contamination or sample mix-up, the assignment of amplified fragment/segments to a compound complete sequence could produce artificial recombinants.
To minimize risk of sample mix-up and contamination, we avoided working with specimens from a given subject and his partner simultaneously.
We also detected a case of sample mix-up due to an erroneous labelling of samples: LL79 was initially labelled as LL80 and vice versa.
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