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Quantification of these populations gives the fractional compositions of each mixed-cell sample (Mix 1 3 in Figure 2 figure supplement 3 ) collected per time point.
Sample mix up issue.
A sample mix proportioning can be found in Ref. (Yang and Lee 2017b).
Tadpoles or A6 cell pellets were snap frozen in liquid nitrogen and homogenized in 100 µl SDS-PAGE sample mix.
After SeptiFast analysis of the 20-microbial-target sample mix, we compared melting temperature (Tm) and peak heights for both extraction methods.
If a standard singleplex PCR approach had been used, there would be a strong suspicion of artificial recombination via sample mix up.
Similar(44)
If there is a sample mix-up, the measurements will not correspond to the correct samples.
Moreover, the MC-MHS-PCR is very rapid, easily implemented, straightforward, and minimizes the risk of sample mix-up and laboratory contamination.
No evidence of sample mix-up or contamination was observed (data not shown).
For the time being, one has to anticipate an omnipresent thin layer of artificial variation incurred by sample mix-up.
There is no reason to assume that other databases, e.g. for animal mtDNA, are less prone to sample mix-up.
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