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These QC samples covered the high (1 sample), mid (1 sample), and low end (2 samples) of the standard curves such that the input was 1.23 × 10 1 ng (high), 1.23 × 10 3 ng (mid), 1.23 × 10 5 ng (low), and 1.23 × 10 6 ng (low) miR-Rx34 per RT reaction, respectively.
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In addition, the sample mid-true boiling points at 20, 50, and 80%% distilled were determined (T b), as shown in Table 2.
To confirm the composition of the sample, Mid-IR spectra of the TPE-An source materials and the as-grown film are compared together, as shown in Fig. 1a.
The dataset was separated into 2 month old samples (YNG) versus 15 month old samples (MID), for 2 states total.
We initially attempted to collect timed overnight urine samples, but switched to spot samples mid-trial.
Research assistants collected the single void urine samples mid-morning through early afternoon using commode inserts.
For the heat-stress-treated samples, mid-L4 animals were heated at 35°C for 4 h and harvested immediately.
For the hypoxia-treated samples, mid-L4 animals were placed in a hypoxic chamber with 0.5% O2 for 4 h and harvested immediately.
For the UV-treated samples, mid-L4 animals were irradiated with 120 J/m UV-C and harvested 4 h post-treatment.
Site 1P was located on a 1st order perennial segment that typically flowed year round, but went dry twice during the summer sampling (mid-August) in 2002 and 2003.
We profiled metabolites in a subset of samples (mid-day on day 14) to characterize metabolic consequences of drought and provide a functional context for our gene expression analysis.
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