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UCPCR could be particularly useful in the developing world where the diagnosis of diabetes is rising fastest and reduced facility and staffing costs associated with a posted urine sample may make large studies easier to do.
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Samples are frozen and kept for eight years, during which they may be retroactively analyzed if a new test is made available.
The using of non-overweight reference sample may makes the proposed blood pressure cutoff points more sensitive to identify children with elevated blood pressure because of with risk factors such as being overweight or obese.
An exploratory component to this research suggested that consumers perceive the concurrent elicitation of hedonic and CATA responses as easy, but that too many samples may make the task tedious.
Dead animals (for example, 'roadkill') or rapidly clotting samples may make blood clots the only DNA-source.
Veterans returning from recent wars with trauma, post-traumatic stress disorder, or other mental health conditions who were not part of this study given its FY2000 sampling may make similar choices in their preferred source of mental health care in the future, because VA is developing more expertise than community mental health providers in these types of treatment [ 49- 51].
In addition, the lack of variance within this sample may have made it more difficult to detect a significant difference in outcome related to either very low or very high endogenous levels.
It is also important to note that urine phosphate measurements were not obtained on a fasting urine sample, which may make measurements less reliable.
Further modifications such as more efficient methods for producing stably transfected cell lines [e.g. ViraPower™, Flp-In™ (Life Technologies)] and robotic sample handling may make the assay suitable for high-throughput screening of miRNA target interactions.
The storage of the biopsies in paraffin could be another possible limitation since this technique might blunt the antigenicity of the samples, which may make it more difficult to detect the receptors.
Such studies have shown, however, that blood may present some technical problems in tissue sampling that may make it an unreliable source of biomarkers this point follows from the fact that the amount of proteins being studied may depend on several factors (such as clotting time).
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