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sample loading.
Overall throughput was limited by sample loading in these cases.
was used to normalize the sample loading.
Reactions were stopped with sample loading buffer.
β-actin immunoreactivity was used to monitor equal sample loading.
GAPDH expression was used to confirm equal sample loading.
Probing with an antibody against GFP comprised a control for plasmid-based expression and sample loading.
Bead-bound proteins were eluted in SDS-PAGE sample loading buffer and heat denatured.
Interacting proteins were eluted with 50 µl 1× protein sample loading buffer.
Sample loading and PCR efficiency were assessed by amplification of GAPDH cDNA.
Amplified human β-actin served as the control for sample loading and integrity.
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