Exact(31)
The optimal sample load was found to be dependent on the paper size.
Sample load is another important factor for obtaining a stable MS signal and accurate quantitative results.
Then, the optimization of flat rectangular and cylindrical panels is carried out for three sample load cases of practical interest.
To achieve a higher sample load capacity compared to miniaturized separation devices for capillary electrophoresis, isotachophoresis is applied.
The functionalities of the method were attested and its analytical conditions, enhanced by using 22% of ACN and 6 min in the sample load step.
Experimental design was used to study the influence of seven extraction variables (sample load, solvents used, solvent ratios, pressure, temperature, extraction time, and rinse volume).
Similar(29)
Column equilibration and sample loading were performed at 600 bar.
Briefly, cells were harvested before boiling in sample loading buffer for 10 min.
Here the shear was due to flow along the capillary axis during sample loading.
But for easy and reliable sample loading, we recommend using the sample loading robot with at least 30 microliters if possible.
Initially, the column was equilibrated with 8 M urea buffer at pH 8 followed by sample loading.
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