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If the sample is strictly peripheral blood, only the ALAS2 (blood) biomarker would be present and the menstrual blood (MMP10) marker would be absent.
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In addition, our sample was strictly treatment-seeking clients.
The size of the sample was strictly related to the search for different profiles.
The pH and the temperature of the investigated samples are strictly monitored and kept within prescribed value ranges.
The carbon samples were strictly degassed under vacuum (0.2 Pa) at 350°C overnight before sorption measurements.
In this context, the vertical sampling was strictly related to species-specific ecology, spatial structure and forest age, being influenced by vertical distribution of trees.
The n-hexane solubilities of the samples were strictly proportional to the volume fraction of penetrable polymer (liquid-like and interfacial components) and the solubility was low in comparison with that of branched polyethylene of the same crystallinity.
Specimen collection and transportation of samples were strictly monitored.
After hybridization, the samples were strictly washed as described by manufacturer and then counterstained with DAPI (Vysis).
A heatmap of the hierarchical clustering (Additional file 2: Figure S6) showed that most of the tissue samples were strictly clustered into their distinctly normal and cancer groups.
These three classes correspond to 'low', 'medium', and 'high' mean survival, where samples are strictly non-cold tolerant (n = 39, <20% survival, <10% standard deviation, s.d)., highly variable/plastic (n = 101, 20 80% survival, ∼20% s.d)., and cold tolerant (n = 48, >80% survival across replicates, and <10% s.d)., respectively (Fig. 2b; supplementary material Table S1).
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