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During the dynamic digestion experiment the substrate is exposed to the movements of the flexible walls [ 8], in the batch model the slurry is stirred [ 7], and in the Megazyme RS method the sample is shaken [ 16].
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Then the sample was shaken vigorously for a few seconds.
The sample was shaken vigorously for exactly 2 min.
The dispersed heap sample was shaken every hour to homogenize the exposure of grains.
Every 2 g of the powdered sample were shaken overnight with 15 ml of demineralized water.
The sample was shaken at 4°C for 15 min in an overhead rotation shaker.
Prior to the analysis, each sample was shaken vigorously using open-air platform shakers to achieve homogeneity.
Thereafter, the sample was shaken by hand, vortexed thoroughly and viable cell numbers determined by drop plating.
Every sample was shaken for 1 min after capping with a PTFE coated septum/aluminum cap and stored into the refrigerator for 10 min before measurement.
To the suspension, Zymolyase 20T was added and the sample was shaken at 50 rpm at 30°C for 40 minutes until the cells were converted to spheroplasts.
The sample was shaken vigorously for 15 seconds.
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CEO of Professional Science Editing for Scientists @ prosciediting.com