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To increase the cobalt content, the calcined sample is re-impregnated with the Co and Zr nitrate solution.
Before the experiments, the sample was re-degassed in situ, followed by cooling to 213 K.
After the H2-TPD, sample was re-oxidized by pulses of 10% Oxygen in helium (TPO).
The acid-insoluble sample was re-dissolved using sodium deuteroxide in D2O diluted to pH 9 with UHQ water.
In this step, the previous value of the current density was halved, and the sample was re-anodized.
After evaporation to dryness, each sample was re-dissolved in 300 µl methanol and used for BPA analysis.
Each sample was re-dissolved in 50 µL of 0,1% trifluoroacetic acid (TFA) and desalted using C18 empore disks.
Each sample was re-extracted and run in duplicate.
Only small between-day variation (4·1 % maximum) was observed when the same sample was re-analysed.
The protein sample was re-suspended in the 6 M urea/100 mM Tris buffer.
This sample was re-examined with the current techniques in the present study.
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