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Last, children in the sample are nested in schools and likely in classrooms, yet only school (not classroom/teacher) identifiers are available from the data collectors.
Both the paraphyletic haplogroups and the clades within which the Ashkenazi Cohen samples were nested, were of clear Middle Eastern origin.
In the AFLP phylogenies, the D. capensis samples are nested within the D. delphis clade.
Similarly, the log-transformed spectral index was modeled as time of collection (daytime or nighttime), and the biological samples were nested within time of collection under the assumption of normality.
The AFLP data support this close relationship, in fact, with the S. longirostris samples being nested within the S. clymene clade, suggesting a recent common ancestry for these two taxa.
The spectral count data on each peptide identified at both time points was analyzed using a Poisson model where the spectral count was modeled with collection time (daytime or nighttime) and the biological samples were nested within the time of collection.
A pre-post intervention two-sample design is nested within the CIS arm to assess the incremental effectiveness of the CIS plus financial incentives (CIS + FI) compared to the CIS without FI on study outcomes.
Additionally, a pre-post intervention two-sample design is nested within the intervention arm to assess the incremental effectiveness of CIS + FI compared to CIS without FI on study outcomes.
Sampling year was nested in site in order to test for local year-to-year variation in infection presence.
Therefore the statistical approach was chosen to take into consideration the multilevel structure of data from our sample where the pen faecal samples (level 1) are nested in herds (level 2).
This study benefited from a relatively large sample size and was nested within a clinical trial that provided a high level of quality assurance.
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