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In general, pre-embedding labeling methods (typically a sample is mildly fixed, permeabilized and labeled by primary reagents before it is embedded and sectioned) can achieve more efficient labeling; post-embedding methods provide better preservation, while the Tokuyasu method often provides the best compromise (Griffiths 1993).
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Next, the sample was mildly washed with autoclaved nanopure water to remove excess stain and minimize background fluorescence.
Each tissue sample was mildly homogenized in an ice-cold Dounce tissue grinder and centrifuged at 700 ×g for 5 min at 4°C.
Samples were mildly agitated for 14 days, and leachates were collected after 6 h, and 1, 2, 4, 6, 8, 10, 12 and 14 days.
Unlike the confocal results, all diffraction samples were mildly agitated (at about 100 rpm using a built-in stir bar) before analysis.
This is mildly unsettling.
Machado is mildly myopic.
Using Clear's interface is mildly revelatory.
The change is mildly radical.
The aroma is mildly bready".
Her exercise tolerance is mildly impaired.
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