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Therefore, when a tumor sample is contaminated by other samples, many somatic-like mtDNA substitutions by contaminants are likely to be overlapped with known mtDNA polymorphisms.
If a sample is contaminated, multiple PCRs will not necessarily clarify the situation.
For example, normalizing to the geometric mean of miRNAs expressed in all samples [ 39] can produce skewed results if a sample is contaminated with miRNAs from blood cells.
A large percentage of unmapped reads (>50%) generally indicates that the sample DNA is of poor quality (e.g., ChIP efficiency is low or the DNA sample is contaminated by DNA from other species).
If one sample is contaminated and contains false intron retentions, such retentions are not expected to be found in other samples; if multiple samples were contaminated, falsely called intron retentions would be found in both tumor and normal samples, which will also be removed by the filter.
Since these two high-throughput sequencings were performed on samples of viral genomic DNA with different purity and concentration, the similarity of the two sequencing results therefore suggests that good results can be obtained even when the sample is contaminated with host DNA as long as there is sufficient coverage for accurate assembly.
Similar(54)
The judges said evidence in the case discounted the idea that the sperm sample was contaminated.
Should a point plot below the line, it could indicate that a particular sample was open to migration of the dating elements or that the sample was contaminated and lay below the isochron when the rock solidified.
A depression of the freezing temperature of a milk sample compared to a reference milk sample indicated that the milk sample was contaminated with a soluble component.
No conventional source water samples were contaminated with resistant E. faecium, whereas one organic source water sample was contaminated with one LIN-resistant E. faecium isolate.
The frozen sputum sample was processed if the first sample was contaminated or had no growth.
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CEO of Professional Science Editing for Scientists @ prosciediting.com