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The sample is applied as a spot near one end of the plate, which is placed (vertically) in a shallow reservoir containing the mobile phase.
Thus, crude peptide sample is applied at overload conditions to extraction columns consisting of SPE tubes containing silica-based reversed-phase packing.
For the free radial-movement boundary condition, the homogeneous sample is applied to a uniform displacement rate on the top boundary so that the deformation is also homogeneous.
The sample is applied on the top of a 5-mm thick layer of a neutral polyacrylamide gel which rests on another 5-mm thick, large-pore polyacrylamide gel which contains positively charged groups.
After pricking a washed and disinfected finger with the kit's single-use lancet, called an "auto-pricker," the blood sample is applied to the tip of the test, which resembles a needleless syringe.
50 μl of this diluted sample is applied to the test well of the device.
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Each sample was applied in triplicate.
The sample was applied on a 15 20% gradient polyacrylamide gel.
7 uL of sample was applied to the overlayer.
The sample was applied to an RNeasy column centrifuged.
After the sample was applied to the column, unbound proteins were removed by extensive washing.
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