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This suggests that when total RNA from a tumor sample is amplified for microarray analysis, the reference RNA should also be amplified from total RNA.
Each sample is amplified in triplicate (3 × cDNA from 100 ng RNA) for TH mRNA and once (1 × 80 ng) for the housekeeping gene β2M.
For PCR, 5 μl of the DNA sample is amplified using a cocktail of the biotinylated PGMY 09/11 and beta-globin primers in a final volume of 100 μl.
Once a clonal marker from the diagnostic sample is amplified by PCR and used in Guthrie card experiments as a positive control, threat of contamination can not be excluded.
Similar(56)
Each tissue sample was amplified with a primer pair including a unique sequence tag.
Each sample was amplified in triplicate.
Each sample was amplified in tetraplicate.
Each sample was amplified in duplicate.
Positive qPCR: duplicates of the sample were amplified by qPCR.
Each sample was amplified in triplicate in two different experiments.
Each sample was amplified in triplicate replication in every experiment.
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