It will be much more difficult to guaranty that other factors are evenly and randomly distributed across the samples, introducing the possibility that metabolic differentiation derives from unknown biases present in the sample set.
Nonetheless, the process of generating simulative samples introduced the noisy data, which decrease the classification accuracy especially for positive samples.
Furthermore, the ability to apply molecular quantification techniques to FFPE samples introduces the possibility of conducting large retrospective validation studies for new molecular diagnostics on well annotated samples with long-term follow-up.
For normal tissue, the problem of heterogeneity might be negligible, but in cancer, that is well known for its heterogeneous set-up, applying different tissue samples introduces the risk that the heterogeneity is behind the expression differences, and not the degradation.
We suggest that the ancestral germplasm of P. abyssinicum was a small sample, introducing a bottleneck into the diversity of this gene pool that still survives today.
However, there was some receptiveness to the idea of at least debating how repeated lapses should be penalised and there was enthusiasm across the sample for introducing the notion of individual responsibility for ensuring best practice hand hygiene.
The difficulty of deconvoluting unambiguously relevant biological events in measurements using a single nanomechanical sensor is highlighted when one considers any sample introduced to the sensor may have a slight variation in temperature/viscosity/refractive index to the buffer fluid.
Incomplete engagement of accessed members of the sample introduces further opportunities for selection bias, which should be recognized and controlled.
Incomplete access to all identified members of the sample introduces multiple opportunities for selection bias, which should be recognized and controlled.
However, cloning to separate different copies of alpha tubulin within each sample introduces greater errors into the sequencing process [ 32].
