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During DBS protocol development, care was taken to ensure an equivalent DBS sample input was used for each serological method, compared to plasma.
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In the verification phase, a sample input is matched against the claimed person's model to produce a score.
The 2D and 3D design spaces showing the combinations of design variables selected as sample inputs are demonstrated in Figs. 14 and 15, respectively.
Briefly, protein samples (Input) were incubated overnight at 4°C with Strep-Tactin Sepharose, and unbound and bound proteins were collected by centrifugation.
Because we must apply these methods many times in the simulation study, we set the dimension of inputs and the number of sampled inputs are small.
Enrichment of the ChIP sample over input was confirmed by UPL-based Q PCR.
A 5 µl sample corresponding to INPUT was collected at this point.
A triangle-based distribution with a median length of 150 bp and 99.0% confidence interval for peak pairs, which were unequal between sample and input, were used.
The mean of 6 assays (variation 0.6 log) was taken as the input RNA amount of the sample, and sample input RNA was normalised accordingly.
The total reads of samples or input were matched to the same size by randomly picking reads.
The robust delay-dependent H∞ control for an uncertain switched system with sampled-data input is investigated in this paper.
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