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Following priming of the IFC in the IFC Controller HX, 5 μl of the cDNA sample+Master Mix were dispensed to each Sample Inlet of the 96.96 IFC.
The sample inlet was situated at a height of 1.0 m above the bowl.
Memory effect was found to be significant for HNO3 especially at high humidity with the long sample inlet tube.
Particle loss in the sample inlet tube was found to play an important role by reducing the number of charged particles entering the denuder.
A tangential filtration unit is attached to the sample inlet line to remove suspended particulate matter and prevent blockage of the micropumps and valves.
Additional increased slip resulted from a rise in the slip to storage ratio, likely due to a shorter effective channel length for NH3 adsorption since most of the P blocked sites at the sample inlet.
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The solution was mixed and 9.5 µL was added to each of two sample inlets.
Individual samples are pipetted into the sample inlets and the gene expression assays are pipetted into the detector inlets.
The chip is mounted on a plastic carrier with interface and containment accumulators and 48 sample inlets and detector inlets.
During this process, pressure is applied to the fluid in the sample inlets and the fluid is pushed into the fluid lines and then into the individual wells.
For samples, the reaction mix contained 2.5 uL 2× Universal Master Mix (Applied Biosystems), 0.25 uL Sample Loading Buffer (Fluidigm Corporation), and 2.25 uL pre-amplified cDNA for loading into the sample inlets of the 48 by 48 Dynamic Array (DA)(Fluidigm Corporation).
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