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600 ng of Cy3-labelled cRNAs were fragmented and hybridized for 17 h at 65°C to each array using the Gene Expression Hybridization Kit (Agilent Technologies) and a gasket slide with a 8 microarrays/slide format for sample hybridization to separate each sample in specific sub-arrays of the 8×15 K format.
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The MCC is a powerful accuracy evaluation criterion for machine learning methods, with unbalanced number of positive and negative samples in specific [30].
By comparing the number of cells in MMC-treated and untreated samples in specific locations, we could infer whether cells were dividing in these locations.
We believe that this ethical aspect is important to facilitate data sharing and communication between professionals about their patients and samples in specific studies.
The reason for this remains unknown, but it could be possible that some of this quarters originally were infected by E. coli which was not present at the time of milk sampling or it could be a result of clustering of samples in specific farms with specific pathogens.
They reported the limitations with regard to selection bias, retrospective data, sample size in specific ages, cross-sectional design, and occurrence of puberty.
Finally, BMI and physical activity variables are correlated, potentially raising the concern of small sample sizes in specific groups crossed by BMI and MMW.
The psychometric test design followed a general application viewpoint where the test was determined by its use, in this case testing individual beliefs of a stratified sample in a specific time and in local specified context.
The assay was measured in duplicate for each sample in the specific time of treatment.
This study is based on one sample in a specific geographical area.
Using a variety of examples, it is demonstrated that the extrema of the failure probability are contained amongst the samples located in specific sectors of the upper level plot as indicated by the hyperbolae.
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