Count samples in scintillation counter via Cerenkov counting.
3. Place dissected samples in scintillation vials containing fixative solution, and vacuum several times to remove air from tissues.
After incubation for 18 24 hr at 37°C in a 7% CO2 humidified incubator, the cells were harvested, and the amount of proliferation was determined by measuring H-TdR uptake, as reflected by the total radioactive counts per sample in liquid scintillation fluid.
Water samples stored in scintillation vials that were not acid-leached were analyzed for Cl-, Br-, F-, NO3-, and SO42- by ion chromatography (Dionex DX500 and a Dionex separation column).
Each sample was placed in scintillation fluid and the radioactivity was measured in a Packard TRI-CARB1 1900CA liquid scintillation analyzer from Packard Instrument Company, Inc. (Downers Grove, IL).
The cells were then washed with ice-cold PBS and each sample was placed in scintillation fluid to measure the radioactivity in a Packard TRI-CARB 1900CA liquid scintillation counter from Packard Instrument Inc. (Downers Grove, IL).
(21, 22) For the efflux study, the cells were treated with 4 μM WHI-P154 and all the samples were placed in scintillation fluid and radioactivity were measured as described previously.
Samples were counted for two minutes per sample in a liquid scintillation counter.
Lobster tissue was collected for SIR analysis at the beginning of the lab experiment by carefully removing the posterior left appendage from each lobster, removing the tissue from the exoskeleton, and freezing each tissue sample in a glass scintillation vial.
Filtered sample was collected and covered in scintillation vials and was analysed for dissolved carbon (DC) and non purgeable organic carbon (NPOC) using TOC-analyzer (TOC-VCSH, Shimatzu).
