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Relative quantification of each amplicon was evaluated in RNA from the tumor samples in biological duplicates each in technical triplicates.
Total RNA from samples (in biological triplicates) that met the criteria were labelled and hybridized to Affymetrix GeneChip Mouse Exon 1.0 ST Arrays.
Equal amounts of aRNA (5.0 μg) of the two samples in biological replicates were reverse transcribed using 0.5 μl of Random Primers (C1181, Promega) and Superscript III reverse transcriptase (Invitrogen) according to the manufacturer's instructions.
The term bioimage comprises all kinds of images generated for biological samples in a biological or biomedical research context using a large diversity of imaging techniques.
All samples are in biological triplicates.
Nevertheless, many potential markers have been described for monitoring EMT in tissue samples and in biological fluids [ 34].
Each sample was tested in biological triplicate.
Each sample was performed in biological triplicate with duplicate analyses in each replication.
Each sample was analyzed in biological triplicate, using individual plants and treatments to test for reproducibility.
Each sample was run in biological triplicate, of which 3 technical replicates were performed.
Each sample was analysed in biological triplicate, using individual plants and treatments to test for reproducibility.
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