Three biological replicates were performed independently from sample collection to phosphopeptide identification using LC-MS/MS.
Gas chromatography coupled to mass spectrometry (GC/MS) was selected as our analytical platform as it allows reproducible measurement, acquisition of a large number of compound peaks from a biological sample, and straightforward peak identification using retention index (RI) and mass spectra by matching to an available reference library [ 31].
Bacterial isolates from environmental and clinical samples were processed for identification using standard biochemical reactions such as oxidase, triple sugar iron, indole, sulfide, motility, citrate and urea hydrolysis.
The extrapolation from the hyperbolic model, fitted onto the average points, obtained by the 10 replications of sampling and reference transcript identification, using reads from the male library only, showed that 7,293 different transcripts were potentially identifiable in the Danio cDNA set (asymptote " a" of the model function).
In this study, we designed 2 pairs of PCR primers for amplifying the cox1 genes of cestodes and nematodes respectively and validated their usefulness for real-time PCR and sequencing identification using clinical samples with cestodes and nematodes collected from a variety of animals and human in 7 countries in Asia, Europe and Africa.
While this problem is less pronounced in protein identification using large samples derived from cell lines where vast majority of proteins are expressed ubiquitously [ 11, 25] this problem is pronounced when small samples are used.
Upscaling of the co-immunoprecipitation (co-IP) experiment allowed protein identification using an EndoH-treated sample (Figure 1B).
Proper sample collection, inoculation on culture media, and final identification using biochemical methods were undertaken.
Identification using various methods classified the tested samples into six taxonomic groups.
Identification using pigmentation alone is not sufficient.
