At the hospital, doctors and nurses orbited my bed, running a flurry of tests: blood samples, heart ultrasound, CAT scan.
In addition, FMDV RNA was not detected in several tissue samples (heart, pancreas, spleen, liver, lung and kidney) from C-S8p260 inoculated mice at 48 hours pi by FMDV RNA quantification (data not shown).
Animals were later euthanized and tissue samples (heart, kidney, liver, lung, muscle, spleen) were obtained.
Other samples (heart) were collected during the same experiment; removal of the heart caused the death of the rats.
Two biological replicates each were tested for myoblasts, skeletal muscle biopsy samples, heart and brain autopsy samples, and leukocytes.
Tissue samples (heart and liver) were obtained and immediately infused with Trizol (TaKaRa) and conserved at −70°C until RNA extraction.
Eight tissues samples (heart, liver, lung, kidney, mammary gland, ovary, uterus and muscle) from each cow were collected within 30 min after slaughter and stored at liquid nitrogen.
Relative quantification of PMCV was employed by qPCR on RNA isolated as described above from selected samples (heart; weeks 0, 2, 4, 6, 8, 9, 10, 11, 12, n = 6, kidney/liver/spleen/PBL/RBC; weeks 4 and 8, n = 6).
Before sampling, heart weight (HW) and left ventricle weight (LVW) were measured in order to be used as cardiac trophism indexes in relation to body weight: HW/BW and LVW/BW.
Correlation coefficients for keratinocytes replicates were: RNAP – 0.79, H3K9me2 – 0.67 and for RNAP ChIP's from liver samples: 0.86; heart samples: 0.83.
