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The precision of estimating a percentile position varies inversely with observation density and, since the majority of distributions have fewer observations at the extremes, extreme observations will be the least precise, whatever the size of the sample generating the distribution.
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We have previously found that the Waters Sep-Pak tC18 removes >99.9%% of the cholesterol present in biological samples generating the oxysterol fraction SPE-1-Fr-1.
The weighted coordinates of these K samples generate the estimated location.
A β-globin control RT-PCR performed on the same samples generated the expected 249-bp product demonstrating the presence of cellular RNA.
MLN collected the samples, generated the data, performed the analyses and wrote the manuscript.
WW isolated samples, generated the SSR and SNP markers, analyzed marker data, and wrote and revised the manuscript.
CC co-designed the study, collected the samples, generated the sequence data, did the analyses and prepared the manuscript.
LL compiled the samples, generated the morphometric and genetic data, and participated in the design of the study.
DR, DCL, AL, EGD, and TWM procured tissue samples, generated the tissue microarray, and performed IHC staining.
DC undertook copy number genotyping and association analysis for the psoriasis samples, genotyping of the control samples, generated the differential bias data and drafted the manuscript.
Of 120 DNA samples, 1 tick, 3 dog, and 3 horse samples generated the predicted band of 573 bp representative of the groEL gene of A. phagocytophilum.
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