Three types of PCR positive controls were included: (i) M. tuberculosis strain H37Rv DNA, (ii) known ZN positive and culture-positive test sample and (iii) positive sample from previous PCR run.
Similarly, three types of PCR-negative controls were also processed in each set of assays: (i) an extraction control (with all the steps but without any extracted material), (ii) a reaction tube with substitution of Qiagen RNase free-water for the test template (iii) a negative sample from previous PCR assay.
He makes this argument by looking at sub-samples from previous surveys.
This footnote was appended on 22 April 2016: Professor Richard Tol disputes a number of assertions made in this article, in particular the claims that he makes his argument by looking at sub-samples from previous surveys (he says his argument is based on whole samples) and that his critique misrepresents previous consensus studies (as earlier results are reproduced exactly).
In contrast, the sequential Monte Carlo methods [25] were constructed to sample from a sequence of related target distributions, using resampling techniques on the samples from previous target density.
The present research investigated relationships among significant asymmetries from seven visuo-spatial and two verbal tasks, combining samples from previous factor analytic experiments with those from three new studies (combined N=789).
We used 108 tree samples from destructive sampling to develop the allometric equations, with maximum tree diameter of 175 cm and another 109 samples from previous studies for validating our equations.
The proposed method is applied to evaluate nanoparticle aggregation/agglomeration in several samples from previous studies including PVC/CaCO3 [30], PCL/nanoclay [31], ABS/nanoclay [32], PLA/nanoclay [33], PET/MWCNT [34], and polyimide/MWCNT [35].
Samples from previous field trips [3], [13], [14] were combined with the newly collected samples for the genetic analysis.
Such a strategy requires instant surveillance data and that PCRlab capacity is devoted solely to testing the samples delivered that day, ignoring any backlog of samples from previous days both assumptions are highly unrealistic.
For this purpose, we used frozen blood samples from previous studies done at our research center.
