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Such a level of throughput would shift the bottleneck of library preparation to sample fragmentation, since nebulization is limited to manual preparations.
Similarly, the improved nature of the detection technology make attention to sample preparation techniques all the more critical as not only sample fractionation but also any sample manipulation including ligations, amplifications, and sample fragmentation can have an effect on the results observed.
This way we can also handle multiple steps of sample fragmentation and purification.
This will result in lower beam intensities due to lower ablation rates coupled with less efficient sample fragmentation that also reduces ionisation efficiency in the plasma.
However, in practice, partially tryptic peptides can also result from the harsh conditions associated with sample processing, sample fragmentation during ionization, or erroneous peptide identification [ 43- 45].
We optimized sample fragmentation, antibody concentration, ChIP conditions, library construction and amplification to generate high quality, high resolution H3K9me3 sequencing libraries from as little as 1,000 embryonic stem cells.
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The relatively high ratio of ECM proteins in CD NP samples, fragmentation of decorin and biglycan, and sparse clusters of cells in the CD samples suggest that, akin to the degenerative human IVD NP, the CD IVD NP represents a naturally occurring degenerative phenotype in contrast to the non-degenerative NCD NP [ 4].
Our results imply that current studies worldwide represent a phylogenetically-conserved sample of fragmentation effects on plant-antagonist interactions.
From 2D SAXS results, it was found that in low octene content sample, lamellar fragmentation occurred resulting in a significant decrease in lamellar lateral size.
Since RNA-seq is a complicated, multiple-step process involving sample preparation, fragmentation, purification, amplification, and sequencing, it is not straightforward to identify and quantify all RNA species from the reads sequenced.
Before hybridization, labeled aRNA samples were fragmented (Fragmentation Reagent Kit, Ambion) according to manufacturer's instructions.
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