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The average 24-hour urine sample for total protein concentration was 187 mg/dL (range, 11-252), and creatinine clearance was 191 mL/min (range, 137-245).
Add reaction buffer to sample for total volume of 100 μl and mix.
A serum sample for total testosterone determination should be obtained between 0700 and 1100 h (Level 2a, A) (31).
All tissues were frozen in liquid nitrogen and equal amounts of homogenized tissues were mixed in a single sample for total RNA extraction.
Intestine from five individuals were combined in a single sample for total RNA extraction and subjected to reverse transcription (TOYOBO, Japan).
Battelle Marine Sciences Laboratory analyzed composite urine samples (10 mL from each daily sample) for total arsenic by inductively coupled plasma mass spectrometry (ICP-MS) (U.S. EPA 1996a) with a method detection limit of 0.2 μg/L.
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Samples for total dissolved iron and cell density measurements were taken from each chamber at prescribed time intervals.
During two years, water samples for total and dissolved metal analyses were collected every third day at both the inlet and the outlet.
High counts (> 3.0 × 102 cfu/ml) were found in 22.5% (n = 49) of all samples for HPC; > 180 mlN/100 ml in 17% (n = 37) of the samples for total coliform and 11.5% (n = 25) of the samples for thermotolerant coliforms.
However, the use of horizontal sampling for total G estimation and indirect estimation of ΔG may reveal as a more effective procedure for a coherent representation of horizontal distribution of trees.
After the sample collection treatment, the reactor was cooled to room temperature using a water bath and sampled for total organic carbon (TOC) analysis of the final effluent in 90-min reaction time.
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