Sample fixation for tissue samples were done in 4% buffered formaldehyde for at least 1 week at 4 °C before shipping to the histology lab.
Areas containing large amounts of necrosis or which had been sampled for tissue microarray construction (most of the male cases) were avoided.
Tissue samples for tissue microarray construction were selected if primary surgery was performed by a gynaecological oncologist from the UMCG between May 1985 and June 2006 and if paraffin-embedded tumour tissue was available.
The time elapsed between surgical tissue removal and freezing of samples for biological characterizations is rarely well defined and/or recorded even for recently stored samples, despite the publications of standard operating procedures for biological sample collection for tissue banks.
Tissue collection and sample selection for tissue microarray (TMA) construction was reported before [ 6].
The median sample size for tissue studies was 60 patients whereas that for serum studies was 89 patients.
Bears representing the non-hibernating condition were still feeding and active when they were euthanized and sampled for tissues between late May and late July (n = 6).
Non-hibernating bears (n = 5) were feeding and active when they were euthanized and sampled for tissues between late May and early October.
Dissected coleoptile and first leaf tissue from 50 100 shoots were combined in one sample for each tissue at both developmental stages.
This article addresses sample design considerations for a national probability sample for human tissue monitoring and specimen banking.
