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Others have adopted this approach in a small sample with success, but called for a larger trial to confirm findings [ 25].
This may be due to differences in laboratory protocols, levels of optimization effort, stringency of criteria for success, sample sizes, and biased selection of well-tested markers in previous studies.
χ analysis was performed on both these sets of data but neither revealed significance at p ≤ 0.05 after Yates' correction for small samples (χ = 5.276 for Success and Impact, χ = 3.537 for Attribution and Impact).
We then compared the multiplex PCR quality assessment across three FFPE breast cancer sample series mentioned in this study, that is, 26 retrospective samples, 93 prospective samples and, the independent study of 1345 breast cancer samples for which PCR success rates were 85, 55 and 94%, respectively.
For Chelex® 100 extracted samples the amplification success for the nuclear gene was even better than for the mitochondrial one.
The overall average sample sequencing success was 75.8 %, ranging from 54.1 % for ITS2 to 95.8 % for rbcLa (Table 2).
Plan for success.
His formula for success?
What made for success?
The "zero" stands for success.
Failure is essential for success.
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