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After the reaction for an indicated period, residual ethanolamine was quantified by withdrawing 50 μL sample for reaction with TNBS in borate buffer of 1.0 mL at 55°C for 15 min. The reaction mixture was cooled with tap water before assay of absorbance.
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A liquid handler was used to collect samples for reaction analysis.
The maximum difference in Kc values among the IFRC <2 mm sediment samples for Reaction 1 is a factor of 2 (Table 1).
Since we needed more detailed quantity of each sample for genotyping reaction, we measured the quantity of DNA using the Quant-iT™ PicoGreen® dsDNA Assay Kit (Molecular Probes, Inc., USA).
The overall sampling rate for reaction or dissociation is thus 0.5 × pon(i ) or 0.5 × poff(i ).
If a sample resulted positive for reaction B, it was assayed again with the same M1-M2 primers, but at 67.5°C or 72°C annealing temperature, which are specific for L. mexicana complex and L. (L).
HγD-Crys samples for multiple reaction monitoring (MRM) mass spectrometry were prepared at 2 mg/mL in 1× reaction buffer and exposed to 2 mW/cm UVR as in the photoaggregation experiments.
Comparison between SAPO-34-U40 SAPO-34-U40 SAPO-34-U404-U100 SAPO-34-U70 SAPO-34-U70 SAPO-34-U70androves same activity of samples for MTO reaction.
We processed each DNA sample in duplicate, and each processing run included samples for background (reaction mixture with all components except SssI enzyme), a hypomethylation control (HeLa cell DNA), and a quality control sample (DNA extracted from a whole-blood sample).
Each DNA sample was processed in duplicate, and each processing run included samples for background (reaction mixture with all components except SssI enzyme), a hypomethylation control (HeLa cell DNA), and a quality-control sample (DNA extracted from a whole-blood sample) to determine the inter- and intraassay CVs (1.8% and 5.3%, respectively).
Three biological replicates were used for each sample and reaction for each test sample was repeated three times.
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