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Harvesting of cells included collection of attached cells following trypsinisation and floating cells, which were combined in the sample for processing.
Harvest 100 ml of the cold sample for OD600 measurements and 100 ml of the [Pi]-labeled sample for processing as described below.
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In other words, although it is possible to use 96-well and even larger assemblages of specimens in conventional Sanger sequencing, it is cumbersome to separate and sort each individual organism into sets of 96 samples for processing.
Even a Fast Fourier Transform FFTT) can be performed over 128 samples in 180 ms, leaving 86% of the sample time for processing.
We thank the Tierseuchenkasse MV for collecting samples, Katja Levin for processing the poultry swabs and the Landesamt für Landwirtschaft, Lebensmittelsicherheit und Fischerei MV for processing samples and the support in planning the study.
The high sampling frequency for processing the travelling wave may block the communications channel and reduce security.
The eventual ellipsoid will serve as a sampling profile for processing the individual intervals with an asymmetric gaussian count profile.
However, the mass of blood, kidney and larvae samples designated for processing using the MagMAX™ kit were often greater than the recommended tissue amount of 5 mg.
Very less amount of sample is required for processing.
Samples were tested for processing time-dependent correlation with gene expression and found to be clear of any technical confounding variables [15].
Two mass spectral libraries were built for this study (Additional files 2 and 3), one for processing samples from the reference solution and another for processing urine samples.
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