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The degree of representativeness of the GEMCAS sample for primary care has been reported in more detail by Moebus et al. [ 11].
Treated sample for primary transcript sequencing and untreated sample for processed transcript sequencing were analysed with the Agilent Bioanalyzer system as described above.
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Even with developmentally matched samples, for primary cell cultures, culture conditions and days in vitro (DIV) also affect the dynamics of gene expression as shown in [ 34].
A small portion of the sample used for primary culture (or a blood sample or DNA derived from the donor) should be frozen or processed immediately.
Using these assumptions the calculated total sample size for primary outcome weight loss was 142 participants.
We used the unrestricted sample for the primary analyses in order to maximize the statistical power.
We think that our population represents a clinically relevant sample for a primary care setting, because all participants experienced leakage of such severity that they actively wanted treatment.
Fourth, we chose to select the sample for our primary analysis based on use of antidementia drugs pre Part D rather than on the presence of a diagnosis code for dementia, as in the sensitivity analysis.
Our results suggest that 10% was the best entire sampling ratio for primary core collection of apricots.
Mouse and rat embryonic samples used for primary cultures were developmentally matched based on the protocol provided by Charles River Laboratories [ 48, 49].
Information on 5'- and 3'-ends of transcripts in sample 1 enriched for primary transcripts and sample 2 enriched for processed transcripts were retrieved from the 454 sequencing data (Table 2) [Additional file 1].
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