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Table 3 Characteristics of the test sample for parallel respirometric and BOD test Wastewater type Physical appearance pH TS (mg/L) DS (mg/L) SS (mg/L) COD (mg/L) NO3 (mg/L) PO4 (mg/L) Food processing industry Milky white, foul odor 8.2 780 370 410 630 30 29.5 Open image in new window Fig. 5 Oxygen uptake profiles of a real wastewater sample run simultaneously with five different dilutions.
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Each of the centers in Catanzaro, Copenhagen and Kuopio sent about 100 fasting and post-glucose challenge plasma insulin samples for parallel analysis to the Tubingen laboratory.
Of the clinical specimens collected from 146 patients, 2 in paired samples for parallel assays by PCR/DNA sequencing and by the GEN-PROBE® PACE® 2C test were found to be "high-positive" for C trachomatis cryptic plasmid DNA by nested PCR/DNA sequencing and also positive by the GEN-PROBE® PACE® 2C kit assay for C trachomatis.
Of these, 16 parallel trials (9 RCT and 7 non-RCT) and 12 pre-post cohort studies reported at least one of the necessary outcome measures and were included in the analysis (pooled sample size for parallel studies = 1,040 [RCT = 474], for pre-post studies = 385).
The eight total RNA samples used for parallel genome-wide transcript profiling (i.e., four secretory-stage samples and four maturation-stage samples) were from the same samples as those used for miRNA expression profiling such that the two data sets (genome-wide transcript and miRNA expression profiling) could be matched with each other.
These quantities differ between experiments because different volumes of sample were processed (some samples were split for parallel analyses) but all samples analyzed satisfied mass balance (initial media Mo = final media Mo + cellular Mo).
A further PCR step was performed to include sample specific barcodes for parallel sequencing using the 454/Roche pyrosequencing platform [35].
In summary we have described an automated high throughput protocol for the preparation of samples for massively parallel sequencing.
Such DNA barcodes for bacterial phyla are not short sequence tags added to PCR products of microbial samples for massive parallel or barcoding pyrosequencing investigations [ 5, 15].
The spectra of non-irradiated samples are identical for parallel and perpendicular polarization.
Each chronic wound site was sampled in duplicate for parallel testing.
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