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The sample for Panel 3 included 200,000 randomly selected service members with 1-3 years of service as of October 2006, with oversampling for women and Marines; enrollment occurred from 5 June 2007 31 December 200808.
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Fold changes between the two panels were calculated based on six pairwise comparisons between labelled samples for panels 'A' and 'B' within each microarray run.
The study population consisted of the entire study samples for Panels 2 and 3 of the Millennium Cohort Study, except those who refused to participate.
We are interested in estimating the true concentration of the molecules in the DNA sample from which we extracted 6 nl×765 = 4.59 µl of sample for each panel.
Briefly, genotypes were obtained for each sample for a panel of 15 unlinked microsatellite markers.
The total reaction volume was 20 μl for each sample (for each panel), containing 4 μl 5X RV16 A (or 5X RV16 B), 4 μl of 8-MOP solution, 4 μl of 5X Anyplex PCR Master Mix (mix well by inverting 5 times) and 8 μl of cDNA product.
The sampling strategy for panel selection was purposive across a number of 'expert' stakeholder groups.
28 40–43 To reduce possible bias, our sampling strategy for panel composition was reflective of the population subgroups under investigation and geographically diverse.
The mean coverage achieved for each sample was 1196 for Panel A and 1309 for Panel B. All detected variants showed a mean coverage of 592 and a mean Qscore of 39 (99.87 % accuracy).
The samples for each panel were prepared in duplicate to assess self-reproducibility.
All samples for this panel were analyzed on FACSCalibur using CellQuest software (Becton Dickinson, Franklin Lakes, NJ, USA).
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