Exact(1)
Briefly, each sample (1.5 μg DNA per sample for parent libraries/0.25 μg DNA per sample for offspring libraries) was digested with SbfI-HF (NEB) (recognition cut site 5′ CCTGCA/GG 3′ and 3′ GG/ACGTCC 5′).
Similar(59)
We evaluated sire offspring relationships for a large sample of offspring from naturally breeding adults in 2006.
All 80 samples of offspring were genotyped for 34 microsatellites that were previously found polymorphic in the captive broodstock and tested on its previous season's spawns populations (Dor et al. 2014).
The heterozygote excess method is generally much less precise than other single-sample estimators (Nomura 2008; Wang 2009) and is best suited for analyzing small populations of species with Type III survivorship for which large samples of offspring are possible (Hedgecock et al. 2007; Pudovkin et al. 2009).
A major constraint for paternity data is that they require extensive sampling of offspring and their parents [6], which can be logistically challenging in many species.
Neither did inverse probability weighting for offspring sex in Sample 1. FRs were slightly lower in analyses not adjusted for parity (Additional file 1 Table 5).
Because of skewed distributions, offspring biomarkers of adiposity were log-transformed when examining their association with maternal PFOA concentrations, and estimates were adjusted for offspring age and timing of blood sample collection.
We calculated this error rate by considering hatchery fish, whose parents were not sampled, as offspring for each parental cohort 2003 2005.
The x point of 5.5 corresponds to analysis of 5 arrays for offspring and 6 for parents (a mean of 5.5 arrays per sample).
Luckily, for offspring too selfish to send their mothers XOX truffles they can't sample themselves, there are plenty of other trendy chocolates to send.
We then drew a simple random sample of 50 individuals from the 5000 offspring and tested the sample for deviation from HWE.
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