Blood samples for liver function and renal function tests were collected to monitor the acute metabolic effects of citrate.
Blood samples for liver function tests were drawn from a peripheral vein after an overnight fast.
The 'starvation' data included samples for liver and skeletal muscle only.
Blood samples for liver biochemistry and venous ammonia level were collected on the day of examination.
Those with recent acute infection also provided further blood samples for liver function testing and confirmatory HEV antibody testing.
Median survival from blood sampling for liver metastasis patients was 234 days (iqr 165 387), with four of the liver metastasis patients surviving at the end of follow-up (25, 28, 32, and 35 months from blood sampling).
Samples for liver enzymes were taken, and sorbitol and lactate clearances performed at points A, B, and D. Following completion of the experiment, a liver biopsy for study of mitochondrial function was taken.
Blood sampling for liver enzyme testing will be performed after run in, and at the 4, 12, 20 and 36 month visits, and at 4, 20 and 36 months for biomarker sampling.
Stigma associated with the testing of blood samples for liver and kidney function during clinical screening for PrEP eligibility was mentioned for fishermen, widows, and serodiscordant couples; participants proposed addressing such stigma by conducting community education about blood draws.
Third, biochemical testing of serum samples for liver indicators that determine whether the study drug should be continued, discontinued or undergo dose modification should be performed in local labs at each investigator site, in order to expedite timely risk management decisions surrounding study subjects.
