During this procedure, a cervical sample for liquid-based cytology and Hybrid Capture (HC) HPV test QIAGEN AG Garstligweg 8 CH-8634 Hombrechtikon, Switzerland) was obtained using a broom type cervical brush and rinsing it in Preservcyt™ buffer solution (Hologic, Inc Bedford, Massachusetts, U.S).
Glycerol hydrogenolysis reactions were performed in a 250 ml stainless steel autoclave equipped with a stirrer, an electric temperature controller and a sample port for liquid samples.
Samples for liquid chromatography tandem mass spectrometry (LC MS/MS) were prepared by incubating wild-type or C333P Shp2 (100 μg, 2.7 μM) with either DMSO or FlAsH (27 μM) for 30 min in Shp2 catalytic domain storage buffer [50 mM 3,3-dimethyl glutarate (pH 7.0), 1 mM EDTA, and 1 mM TCEP].
Miniaturized sample preparation methods designed as the sample pretreatment for liquid phase separations, such as liquid chromatography, capillary electrophoresis and capillary electrochromatography, have been reviewed especially for the on-line coupling of the sample preparation process and the separation process.
The new transmission mode desorption electrospray ionization (TM-DESI) technique is not applicable to bulk materials, but instead is a method designed to simplify the sample preparation process for liquid samples and sample extracts.
Sample preparation for liquid ICPMS analysis was done using laboratory water type 1 and nitric acid of reagent grade (EMSURE ISO, Merck, Darmstadt, Germany) which were further purified in-house by using a sub-boiling distillation system (Milestone Inc., Shelton, CT, USA).
Conversely, totally dissolved samples for liquid-state NMR spectroscopy contain molecules that tumble isotropically, thus fully minimizing the anisotropicity of solid matter and enabling acquisition of high-resolution NMR spectra.
The first sample for routine liquid based cytologic examination and the second sample for FACS analysis were immersed in 10 mL of phosphate buffered saline (PBS, Gibco Laboratories, Grand Island, NY, USA) and Dulbecco's modified Eagle's minimal essential medium/F12 medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% defined fetal bovine serum (FBS) (Invitrogen), respectively.
This means that it is hugely beneficial to design an actuator so that the electric field is parallel to the sample, which for liquids is most easily accomplished by drawing them into a thin cylinder.
HPV DNA testing, determined from an aliquot of the same sample used for liquid-based cytology, is also widely used as a 'reflex' test to triage patients with ASCUS, thus eliminating the need for a repeat office visit.
