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Before extraction, 17 C-labeled PCDD/PCDFs and 18 C-labeled PCBs congeners were added to each sample for internal standard calibration and quantification by the isotope dilution method.
Samples from 153 patients were brought to the NRL between March and September 2009 for culture, with 39 of the patients providing more than one sample for internal control, but these duplicate samples were not considered in the final statistical analysis.
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A synthetic spike-in control, Caenorhabditis elegans miR-39 (cel-miR-39), was added to the lysed samples for internal normalization.
In every 96-well plate for assay, there is one well for blank control and five wells for duplicate check on five samples for internal quality control.
This gave convergent validity (n = 61), test-retest reliability (n = 36), discriminant validity (n = 52) and the pooled samples for internal consistency and factor analysis (n > 100) [ 31].
A high-pressure Hg lamp (HB400X-15 400 W; SEN LIGHTS CORP) in a quartz water-cooling jacket was placed at the centre of a 500-mL reaction vessel containing the sample solution for internal irradiation.
Insulin was added to the sample for an internal standard.
Thus, our study employed a split-sample approach for internal validation.
Per a court filing, Exygen discussed the cause as a problem of initially prepared samples used for internal calibration [see Supplemental Material, Note 7 (doi:10.1289/ehp.0800379.S1)].
On each well plate, a control plasma sample was included for internal quality control purposes.
To define the limit of detection for this DNA resequencing platform, we screened 90 MDS samples for FLT3 internal tandem duplications (ITD) using standard agarose gel electrophoresis and the Agilent LabChip.
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