Working at the Human Immune Monitoring Center, team members are searching 600 blood samples for infectious microbes, inflammation-related molecules and genetic flaws.
We therefore tested a subset of real-time RT-PCR positive excRT-PCR positivefor infexcretionirusamples
Consent to test the samples for infectious diseases affecting the blood supply was obtained from the blood donors as part of the Donor Health Assessment Questionnaire Form.
In addition, we also tested WBC from these samples for infectious organisms using an in vitro macrophage monolayer system, rather than the non-phagocytic HEp-2 or HeLa cells often used by others.
As part of the examination process, researchers put each patient in a room and then collected air samples, looking for infectious particles.
The supernatants from these samples were frozen at –80°C until samples were titrated for infectious units.
Stool samples were negative for infectious organisms.
Samples were tested for infectious vaccinia virus by inoculation of fluid cultures of Vero cells grown in 12-well plates.
We would like to thank Dr. Rafick Pierre-Sekaly, Bader-Yassine Diab, Dr. Jean-Pierre Routy and Dr. Mohamed-Rachid Boulassel for providing samples of infectious plasma and leukapharesis material.
Therefore we might have included a non-representative sample of statin trials for infectious outcomes.
