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Cryo-ultramicrotomy sectioning of vitrified materials is a conventional method to acquire the sample for electron microscopic study, but this method inevitably suffers from distortions and deformations caused by the mechanical cutting process, and forms unavoidable sample compressions in the cutting direction, which introduce significant artifacts to the later ET analysis.
The grids with sample for electron microscopy were prepared as described earlier (Fernández et al., 2014).
A tissue sample recovered from a paraffin-embedded sample for electron microscopy, showed round and oval rickettsia-like microorganisms, maximum diameter 0.2 0.5 μm, inside the cytoplasm endothelial cells.
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The samples for electron microscopic studies were prepared as shear of sintered tablets.
Samples for electron microscopy analysis were prepared by dropping the diluted colloidal solution onto copper grids covered with a thin (approximately 5 nm) continuous amorphous carbon film and allowing the solvent to evaporate.
Samples for electron microscopy were fixed in cacodylate-buffered glutaraldehyde (4.5%, pH 7.3), postfixed in osmium tetroxide (1%) and embedded in Spurr's resin.
To prepare NB samples for electron microscopy and spectroscopy analyses, the cell culture medium of a flask containing a 1-month-old culture of NB was discarded and the NB sample which consisted of a white precipitate adherent to the flask was scraped using a sterile cell scraper (Corning).
Nucleoprotein complexes were prepared according to the manufacturer conditions by incubating RecA protein (10 µM) with φX174 ssDNA (15 µM) in buffer containing 50 mM Tris-HCl, (pH 7.5), 5 mM MgC12, and 50 mM NaCl at 37°C for 30 min. Samples for electron microscopy were stained with were 2% methylamine tungstate (MT), pH 6.8 (Nano-W, Nanoprobes Inc). to keep pH close to physiological conditions.
There are several ways to prepare samples for electron microscopy.
Samples for electron microscopy were prepared as described previously (van der Wel et al., 2007).
There are several ways to prepare biological samples for electron microscopy.
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