A form for indicators covering demographics, health, nutrition, water and sanitation, education, child development and women's empowerment was created and submitted to each of the five sample districts for completion ahead of the field survey.
Most Children's Centers conducted repeated measures of environmental exposures, including inspection, settled dust sampling, and air sampling; completion rates for these evaluations ranged from 73 to 91%.
Two samples had insufficient sera for completion of the I-ELISA test, so the final data set was for 1,375 animals.
The samples were transferred to Glasgow for completion of leptin and insulin assays.
The survey took approximately 25 minutes and all participants in the random and non-random sample received a $20 gift card for completion of the telephone survey.
> -wrap-foot> ARI was defined as occurrence of a new onset or worsening of one or more of the following five symptoms: cough, sore throat, nasal congestion, earache or discharge, breathing difficulty The quality of collected surveillance data is checked by health supervisors who revisit 10%% sample of the surveyed households, check for completion of forms in the field.
Minimum 2 h incubation of samples in reaction mixture was required for completion of the reaction.
For completion of the three genomes, DNA samples were submitted for pyrosequencing analysis (454 Life Sciences, Branford, CT).
DNA and RNA samples were stored at −80 °C and shipped to Illumina, Inc. (San Diego, CA) for completion of assays.
Cases and controls receive a financial compensation for their participation: 20 euros for completion of the interview and 40 euros for participation in both blood sampling and completion of the interview.
